Abstract—We designed high-throughput microchip which detects single nucleotide polymorphisms (SNPs). The chip consisted of DNA extraction part and SNP detection part. DNA was extracted by lysis buffer, and then amplified by polymerase chain reaction (PCR). In SNP detection part, CEL I endonuclease selectively cleaved mismatched dsDNA, so wildtype DNA was distinguished from mutanttype. Sig
and xicS genes. Homologous recombination is type of genetic recombination which DNA sequences are exchanged between two similar or identical strands. It can be used for deletion of the target gene.
First, design and fabricate the similar DNA construct you want to insert into the chromosome in place of the wild-type allele. This constructs may contain selective marker that help isolate the kn
◉ Stringent Response
- Stringent Response란 세포가 서식하는 환경이 급격히 변하게 되거나 어떠한 요인에 의해서 세포의 생존에 위기가 닥쳤을 시에 이런 위급한 상황에 대처하기 위해서 세포내의 대사를 변화시키는 작용을 말한다. 이 작용을 통해 세포가 위기상황에서도 그 변화에 적응해 나가는 능력이
Abstract
Bioethanol is one of the alternative sources of oil resources which received attention with biodiesel. Many countries produce bioethanol from food sources such as corn and sugarcane, but it causes food-energy conflicts and farmland problem. So it needs to focus on new biomass which is sustainable, renewable, cost-effective and do not occurred any conflicts. Algae are one of the bioma
Gene Information from Arabidopsis.org
3) Dimunitia(DIM) 에 의한 애기장대의 표현형(Phenotype)
4) 식물생리학 7조에 의한 애기장대 표현형 관찰결과
Result) 식물생리학 7조가 관찰한 애기장대 표현형은 AT3G19820 염색체 위에 있는 T-DNA transformed mutant lines인 Dimunitia 라는 Mutant 로써 이 Mutant의 표현형은 훨씬 짧은 h
Lipopolysaccharides (LPS), also known as lipoglycans,
are large molecules consisting of a lipid and a polysaccharide
joined by a covalent bond; they are found in the outer
membrane of Gram-negative bacteria, act as endotoxins and
elicit strong immune responses in animals.
LPS is the major component of the outer membrane of
Gram-negative bacteria, contributing greatly to the structu
다. Amino Acid의 Titration과 pH buffer 작용
Amino Acid를 NaOH와 같은 염기로 Titration을 하여 얻어지는 Titration Curve는 적정하는 Amino Acid의 pH buffer 작용을 잘 설명해 준다.
즉 Amino Acid의 Carboxyl기는 Weak Acid이므로 NaOH-와 같은 Strong Base와 반응하면 일정한 pH 범위 내에서 큰 pH 변화를 일으키지 않는 pH Buffer 작용을 한
Amino Acid의 Titration과 pH buffer 작용
Amino Acid를 NaOH와 같은 염기로 Titration을 하여 얻어지는 Titration Curve는 적정하는 Amino Acid의 pH buffer 작용을 잘 설명해 준다.
즉 Amino Acid의 Carboxyl기는 Weak Acid이므로 NaOH-와 같은 Strong Base와 반응하면 일정한 pH 범위 내에서 큰 pH 변화를 일으키지 않는 pH Buffer 작용을 한다는
Introdction
HELICOBACTER PYLORI -> chronic inflammatory, intestinal metaplasia, dysplasia, and finally adenocarcinoma
cag pathogenicity, which encodes a type IV secretion system (T4SS)
-> more severe inflammation, gastric cancer
proinflammatory + mitogenic stimuli -> COX-2 -> 위 상피세포 -> PGE2 -> 암
PGE2 -> tumor growth, angiogenesis, inhibiting apoptosis, inducing invas